Formerly Covance Antibody Products
Product Index, A-Z
Microtubule Associated Proteins
Spectrin Binding Protein
Myelin Basic Protein
Nuclear Pore Complex 153
Blood Brain Barrier
Tumor Necrosis Factors
Thyroid Stimulating Hormone
Pattern Recognition Receptors
Amyloid Beta C-Terminal mAbs
Amyloid Beta Central mAbs
Amyloid Beta N-Terminal mAbs
Amyloid Beta Peptides
Amyloid Beta Rodent mAbs
Amyloid Beta-Modifiying Protein
HRP USA Detection
Microtubule Associated Proteins
Interactive APP Diagram
Terms & Conditions
BRST-2 (GCDFP-15) Monoclonal Antibody, Purified
Neuronal Class III β-Tubulin Polyclonal Antibody, Purified
SNAP-25 (SMI 81) Monoclonal Antibody
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IMAGES & PROTOCOLS
Pan-Axonal Neurofilament Marker (SMI 312) Monoclonal Antibody
Catalog Number SMI-312R
Monoclonal Antibody against Pan-Axonal Neurofilament Marker
This format of clone SMI 312 will be discontinued Dec 2015.
A purified format will be launched but currently not available.
**Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.
SMI 312 has been selected to provide a specific marker for axons in tissue sections and cultures. In contrast to individual antiphosphoneurofilaments that identify different subsets of neurofilament phosphoepitopes, and are, therefore, especially suitable for defining functional and regional differences in normal and pathologic axons, SMI 312 is a convenient marker for axons in general. SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is, thereby, useful in establishing early connectivities with SMI 311, antinonphosphoneurofilament-identified dendrites. SMI 312 visualizes aberrantly sprouting axons in neuritic plaques derived from cortico-cortical fibers in Alzheimer's disease and identifies loss of synaptic circuitry proposed to be the basis of memory
Neurofilament H Non-Phosphorylated (SMI 32) Monoclonal Antibody, Purified
Cat No. SMI-32P
GFAP (SMI 22) Monoclonal Antibody Cocktail
Cat No. SMI-22R
GFAP (SMI 26) Monoclonal Antibody Cocktail
Cat No. SMI-26R
GFAP (SMI 21) Monoclonal Antibody
Cat No. SMI-21R
Neurofilament H Phosphorylated (SMI 34) Monoclonal Antibody
Cat No. SMI-34R
Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody
Cat No. SMI-35R
Protocol: IHC using Sternberger Monoclonals
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and ELISA.
Suggested Working Dilution
The extent of permissible dilution of SMI 312 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
: Formalin-fixed, paraffin-embedded tissues & frozen sections
: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 3 pH4.8 (SIG-31900).
: 60 minutes at room temperature with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Positive Control: human cerebellum tissue
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
03/11/2015 12:00:00 AM
Choi Y, Nardo A, Kramvis I, Meikle L, Kwiatkowski D, Sahin M, He X. Tuberous sclerosis complex proteins control axon formation.
Genes & Dev.
; 22: 2485 - 2495, 2008
Bussière T, Bard F, Barbour R, Grajeda H, Guido T, Khan K, Schenk D, Games D, Seubert P, Buttini M. Morphological characterization of Thioflavin-S-Positive amyloid plaques in transgenic Alzheimer mice and effect of passive Abeta immunotherapy on their clearance.
Am J Pathol 165
Chung RS, Vickers JC, Chuah MI, West AK. Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury.
J Neurosci 23
Soifer D, Nicoletti V, Cabane K, Mack K, Poulos B: Expression of neurofilament protein NF-H in L cells.
J. Neurosc Res 30
Pestronk A, Watson DF, Yuan CM: Neurofilament phosphorylation in motor axons with high and low growth potential.
J Neurochem 54
Sternberger LA, Harwell LW, Sternberger NH: Neurotypy: Regional individuality in rat brain detected by mmunocytochemistry with monoclonal antibodies.
Proc Natl Acad Sci USA 79
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