Formerly Covance Antibody Products
Product Index, A-Z
Microtubule Associated Proteins
Spectrin Binding Protein
Myelin Basic Protein
Nuclear Pore Complex 153
Blood Brain Barrier
Tumor Necrosis Factors
Thyroid Stimulating Hormone
Pattern Recognition Receptors
Amyloid Beta C-Terminal mAbs
Amyloid Beta Central mAbs
Amyloid Beta N-Terminal mAbs
Amyloid Beta Peptides
Amyloid Beta Rodent mAbs
Amyloid Beta-Modifiying Protein
HRP USA Detection
Interactive APP Diagram
Terms & Conditions
Neuronal Class III β-Tubulin (TUJ1) Monoclonal Antibody, Purified
C219 Monoclonal Antibody, Purified
Green Fluorescent Protein (GFP) Monoclonal Antibody, Purified
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IMAGES & PROTOCOLS
Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, Purified
Exclusively Provided by BioLegend
Catalog Number SIG-39220
Beta Amyloid (Aβ) Monoclonal Antibody, 4G8, Purified
(Formerly Signet Catalog Nos.: 9220-02, 9220-05, 9220-10)
BioLegend is the Exclusive Provider of this Antibody Product
Also Known As
amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide
**Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.
Protein G Purified Antibody (in PBS; no preservatives or carrier proteins)
This antibody is reactive to amino acid residues 17-24 of beta amyloid. The epitope lies within amino acids 18-22 of beta amyloid (VFFAE). Beta amyloid forms are deposited in the CNS of patients with Alzheimer’s disease and Down’s syndrome. Signet’s beta amyloid antibody 4G8 reacts to the abnormally processed isoforms, as well as precursor forms.
Beta Amyloid (1-8) Monoclonal Antibody, Purified
Cat No. SIG-39110
β-Amyloid (20.1) Monoclonal Antibody, Purified
Cat No. SIG-39350
Beta Amyloid, 1-16 (6E10) Monoclonal Antibody, Purified
Exclusively Provided by BioLegend
Cat No. SIG-39320
Beta Amyloid, Rodent Specific Monoclonal Antibody, Purified
Cat No. SIG-39155
Beta Amyloid, Rodent Specific Polyclonal Antibody, Purified
Cat No. SIG-39153
Protocol: Western blot using Beta Amyloid antibodies
Protocol: IHC using Beta Amyloid antibodies
APP Processing and Product List
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification (IP) and ELISA.
Suggested Working Dilution
The optimal working dilution should be determined for each specific assay condition.
1:100 - 1:1,000*
1:500 - 1:1,000
: Formalin-fixed human and animal paraffin-embedded brain sections, frozen tissue sections
: Formic acid (70%) for 10-30 minutes at room temperature
1:250 - 1:500
*Expected MW: APP= 100 kD, Beta Amyloid = 4kD
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
08/29/2014 11:00:00 AM
Thakker DR, et al. Intracerebroventricular amyloid-beta antibodies reduce cerebral amyloid angiopathy and associated micro-hemorrhages in aged Tg2576 mice.
Proc Natl Acad Sci USA Feb 25
Kimura N, et al. Age-related changes of intracellular Abeta in cynomolgus monkey brains.
Neuropath Appl Neurobiol 31(2)
Klyubin I, et al. Amyloid beta protein immunotherapy neutralizes Abeta oligomers that disrupt synaptic plasticity in vivo.
Nat Med 11(5)
Iijima K, et al. Dissecting pathological effects of human Abeta40 and Abeta42 in Drosophila: a potential model for Alzheimer’s disease.
Li M, et al. Macrophage colony stimulatory factor and interferon-gamma trigger distinct mechanisms for augmentation of beta-amyloid-induced microgliamediated neurotoxicity.
J Neurochem 91
Poduloso JF, et al. Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer’s disease amyloid plaques.
Venezia V, et al. Apoptotic cell death influences the signaling activity of the amyloid precursor protein through SchA and Grb2 adaptor proteins in neuroblastoma SH-SY5Y cells.
J Neurochem 90
Kim KS, et al. Production and characterization of monoclonal antibodies reactive to synthetic cerebrovascular amyloid peptide.
Neurosci Res Comm 2
Kim KS, et al.
Neurosci Res Comm 7
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